— 04 — FISHERIES, AQUACULTURE & AQUAPONICS

From Treating Water to Designing Water.
Closed-Loop RAS & Aquaponics via Shelltas Water OS

Traditional Recirculating Aquaculture Systems (RAS) and aquaponics are plagued by heavy water exchange costs, pathogen surges, and dependency on chemical disinfectants and antibiotics. Shelltas Water OS harnesses SeaFect natural artificial seawater to imbue water with autonomous self-stabilization, delivering zero water exchange, zero antibiotics, and sustainable food production.

10+ Mos
Continuous Zero Water Exchange

Sustained zero-exchange water quality balance verified in 5-tank system.

0 Chemicals
Zero Antibiotics & Biocides

Stimulates biological immunity without chemical dosing or UV irradiation.

6 Days
Sardine Sashimi Shelf Life

Doubled sashimi edible shelf life from 2–3 days to 6 full days.

Global Summits
London & Singapore 2026

Featured startup at Blue Food London & oral paper at World Aquaculture Society.

LAND-BASED RECIRCULATING AQUACULTURE ARCHITECTURE

Land-Based RAS System Architecture & Flow

Diagram of closed-loop RAS combining Water OS microbial nitrification and SeaFect mineral buffering.

Recirculating Aquaculture System (RAS) Architecture

Autonomous Water Circulation & Buffering Flow

Closed-loop biological system maintaining pristine aquatic water through natural marine minerals and microbial ecology.

✓ Zero-Exchange Verified
NODE 01Fish Tank

Culture Tank

Eel, shrimp, medaka, chub, crab, abalone.

Organic NH₃ Output
NODE 02Alkalinity

SeaFect Alkalinity Buffer

Replenishes alkalinity consumed by biological activity, preventing sharp pH drops.

Alkalinity Buffer & Stable Habitat
NODE 03Nitrogen Cycle

Microbial Nitrogen Cycling

Synergy with microbiome prevents NH₃/NO₂⁻ buildup with ultra-low nitrate accumulation.

Suppressed Nitrogen & Denitrification Test
NODE 04Recirculation

Clean Water Return

Purified mineral-rich water flows continuously back to culture tanks.

Zero Antibiotics / 99% Water Saved
FLOW LOOP:Culture Tank ➔ Separation ➔ SeaFect Alkalinity ➔ Nitrogen Cycle ➔ Recirculation ↻
CONTINUOUS 24/7 CLOSED CYCLE
STEP 01Feeding & Excretion

Culture Tank (Aquatic Ecosystem)

Fish (eel, medaka, pale chub, tropical fish), crustaceans (shrimp, mitten crab), and abalone inhabit the tank. Feeding produces ammonia and organic waste.

Key Performance Metric
High DO & Innate Vitality
STEP 02Alkalinity Replenishment

Mineral & Alkalinity Replenishment by SeaFect

Minerals derived from SeaFect are supplied to the water, replenishing alkalinity consumed by nitrification and biological activities. Suppressing abrupt pH fluctuations, it supports the maintenance of an aquatic environment conducive to the activity of aquatic organisms and microorganisms.

Key Performance Metric
Alkalinity Replenishment / Stable pH Buffer
STEP 03Nitrogen Cycling

Microbial Nitrogen Cycling

The mineral environment formed and maintained by SeaFect interlocks with the microbiome established on filter media and substrates. Suppresses accumulation of nitrogen compounds such as ammonia and nitrite, stabilizing water quality. Extremely low accumulation of nitrate has been observed even under long-term operation, and nitrogen removal pathways including denitrification are currently being verified.

Key Performance Metric
Ammonia/Nitrite Suppression / Ultra-low Nitrate
STEP 04Zero Water Waste

Clean Water Recirculation Loop

Mineral-rich clean water cycles back into culture tanks. Eliminates massive fresh water exchanges and expensive pharmaceuticals, maintaining biological balance.

Key Performance Metric
99% Water Saved / Antibiotic-Free
THE 4 ZEROS OF WATER OS

The 4 Zeros: Transforming Conventional RAS

Moving away from forceful mechanical and chemical water treatments. By designing water quality naturally through marine minerals and microbial ecology, Water OS eliminates conventional CapEx burdens and biological disease risks.

01. Zero Water Exchange99% Water Saved

Eliminates daily 10–30% heavy water flushes. Enables sustainable closed-cycle aquaculture in arid, inland, and urban environments.

Daily water exchange: conventional RAS 10–30% daily, Water OS 99% water saved
02. Zero AntibioticsAntibiotic-Free

Protects stock not via chemical dosing, but by fortifying mucosal barriers and innate immunity using SeaFect bio-minerals, preventing drug-resistant pathogens.

03. Zero Chemicals / BiocidespH 7.8–8.2 Buffer

Eliminates chemical disinfectants and manual soda-ash buffering. Autonomous mineral dissolution maintains pH 7.8–8.2 and biological equilibrium.

04. Zero UV / Ozone EquipmentLower CapEx & OpEx

Eliminates expensive UV irradiation lamps, ozone generators, and giant filters, dramatically reducing initial plant CapEx and ongoing electrical costs.

Closed loop aquaculture with Water OS
Water OS Closed-Loop Pilot Aquarium SystemSHELLTAS LAB VALIDATED

What is SeaFect Natural Artificial Seawater?

Proprietary formula compounding porous bio-calcium from upcycled shells with marine trace elements. Perfectly replicates natural seawater mineral balance while eliminating coastal viral contaminants and red-tide risks.

EMPIRICALLY VALIDATED SPECIES

Empirically Validated Aquatic Species

Proven empirical stability across sensitive fish, molt-dependent crustaceans, and shell-forming bivalves.

Illustration of Japanese Eel
Japanese Eel
Illustration of Minami-Numaebi (Shrimp)
Minami-Numaebi (Shrimp)
Illustration of Medaka (Rice Fish)
Medaka (Rice Fish)
Illustration of Abalone
Abalone
Illustration of Tropical Fish
Tropical Fish
Illustration of Oikawa (Pale Chub)
Oikawa (Pale Chub)
Illustration of Mokuzugani (Mitten Crab)
Mokuzugani (Mitten Crab)
Illustration of Marine Prawns
Marine Prawns
Japanese EelMinami-Numaebi (Shrimp)Medaka (Rice Fish)AbaloneTropical FishOikawa (Pale Chub)Mokuzugani (Mitten Crab)Marine Prawns
01. SPECIES

Japanese Eel

10+ Months Zero-Exchange

Highly sensitive to ammonia. Synergistic nitrification and SeaFect buffer neutralize 99.9% ammonia, securing >98% survival without antibiotics.

Survival Rate: 98.4% / Meds: 0
02. SPECIES

Freshwater Shrimp (Numaebi)

In-Tank Breeding & Molt Success

Fragile freshwater shrimp vulnerable to water shifts. Ionized Ca²⁺ supports exoskeleton cuticle hardening, preventing molt failures and enabling breeding.

Breeding Established / Molt Support
03. SPECIES

Medaka (Japanese Rice Fish)

Long-term Zero Exchange & Spawning

Prevents pH crash mortality for fry and breeders. Autonomous mild-alkaline buffering increases egg yield and fry survival rates without water shifts.

High Fry Survival / Continuous Spawning
04. SPECIES

Abalone & Shellfish

Shell Bio-Calcium Recirculation

Upcycled scallop minerals provide bio-available Ca²⁺ for shell calcification, reducing abalone mortality in recirculating tanks.

Shell Upcycling / Mineral Reflux
05. SPECIES

Tropical Ornamental Fish

Shock Prevention & Fin Protection

Eliminates water replacement osmotic shock and fin rot caused by acid dips. Instant ammonia breakdown maintains vivid coloration and vitality.

Mucus Protection / Zero Shock
06. SPECIES

Oikawa (Pale Chub)

Pristine Stream Fish Stability

Riverine fish requiring high dissolved oxygen and clean water. Activated aerobic nitrifiers maintain crystal-clear, dynamic river-grade water.

Clear Water Quality / Zero Organics
07. SPECIES

Mokuzugani (Mitten Crab)

Molt Hardening & Cannibalism Drop

Prevents post-molt softening and limb loss. Rapid calcium intake accelerates carapace hardening, supporting dense closed-tank co-habitation.

Carapace Hardening / High Density
08. SPECIES

Kuruma & Vannamei Prawns

Pathogen Suppression in Marine RAS

Targeted for disease-prone marine prawns. Synthetic marine water enriched with SeaFect eliminates stress and allows antibiotic-free closed farming.

Natural Artificial Seawater / Zero Meds
AQUAPONICS & HYDROPONIC SYMBIOSIS

Aquaponics & Hydroponic Symbiosis with Water OS

Harnessing fish metabolic byproducts to nourish hydroponic crops while plants purify water back to fish tanks. Shelltas Water OS autonomously balances water chemistry, delivering pesticide-free and chemical-free dual food production.

01. CYCLE

Total Nitrogen & Ammonia Recycling

Nitrifying flora convert raw fish ammonia into plant-accessible nitrate nitrogen. Leafy greens, herbs, and tomatoes absorb nutrients and return cleansed water to tanks.

Zero Effluent // Fertilizer Input Cut
02. BUFFERING

Preventing Acid Crashes & Mineral Supply

Prevents acidification crashes caused by nitrification while infusing bio-calcium and trace minerals, bolstering root architecture and photosynthesis.

Autonomous pH 6.8–7.4 // Ca Supply
03. ZERO CHEMS

Pesticide-Free & Antibiotic-Free Harmony

Overcomes the core dilemma where antibiotics wither crops and synthetic pesticides kill fish, achieving authentic certified organic quality.

Antibiotic-Free // Chemical Pesticide-Free
04. PLACEMENT

Urban Vertical Farms & Extreme Environments

Zero external runoff and minimal make-up water make it ideal for urban commercial buildings, repurposed schools, desert outposts, and island micro-grids.

Deployable Anywhere Inland & Arid
LOGISTICS & COLD-CHAIN INNOVATION

Seafood Cold-Chain Breakthrough: Extending Sashimi Shelf Life to 6 Days

Shelf-life limits determine the geographical boundary of fresh seafood logistics. SeaFect extends sardine sashimi preservation from 2–3 days to 6 full days.

Sardine sashimi shelf life: standard preservation 2–3 days, SeaFect preservation 6 days
Empirical Trial: Highly Perishable Sardine Comparison

Retains Premium Raw Sashimi Quality after 6 Days

Unlocks long-range domestic surface transit and international air/sea exports previously restricted by rapid freshness degradation.

Standard Preservation
2–3 Days
Discoloration & Spoilage
SeaFect Preservation
6 Days
Preserves Sashimi Freshness
Alignment with Cold-Chain Modernization Policies

Complies with national food distribution optimization programs, preventing origin discard waste while improving energy efficiency in cold chains.

EXPORT READY // COLD-CHAIN LONGEVITY
GLOBAL VALIDATION PARTNERSHIP // SINGAPORE AIC

Empirical Partnership with Singapore’s Aquaculture Innovation Centre (AIC)

In Singapore—a global epicenter for high-density tropical aquaculture—Shelltas collaborates with the Aquaculture Innovation Centre (AIC), supported by Enterprise Singapore and the Singapore Food Agency (SFA). This partnership evaluates SeaFect for closed recirculating systems (RAS), antibiotic-free prawn farming, and microbial nitrogen cycling under rigorous tropical conditions.

Supported by Singapore Government Agencies & AIC

Aquaculture Innovation Centre (AIC) × Enterprise Singapore × Singapore Food Agency (SFA)

Aligned with Singapore "30 by 30"
01. SHRIMP & MARINE RAS

Vannamei Prawn Survival & Antibiotic-Free RAS

Providing pre-ionized bio-calcium, magnesium, and trace marine minerals for disease-sensitive Whiteleg shrimp (P. vannamei). Eliminates osmotic shock and molt deformities, validating dense closed farming without antibiotics.

Zero Antibiotics // Rapid Molting // High Survival
02. WATER CHEMISTRY

Alkalinity Buffering & Nitrate Suppression

Autonomously replenishes alkalinity consumed by biological nitrification, preventing fatal pH crashes. Jointly investigating unique low nitrate accumulation phenomena and microbially-mediated denitrification pathways under long-term closed circulation.

Autonomous pH 7.8–8.2 // Nitrogen Cycle Validation
03. GLOBAL FOOD SECURITY

National Resilience & Global Scaling

Directly aligned with Singapore’s national "30 by 30" food security strategy. Establishing a global technological benchmark for low-water closed farming scalable across Southeast Asia and resource-constrained regions worldwide.

Zero Water Waste // Cross-Border Expansion
GLOBAL FOOD SECURITY & MARS MISSION

From Terrestrial Food Crises to Sustainable Food on Mars

Amidst global seafood supply shortages, closed-loop aquaculture requiring minimal water input is a critical global infrastructure. Shelltas Water OS is designed for extreme resource-constrained environments on Earth and extraterrestrial closed ecological life-support systems (CELSS) on Mars.

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Blue Food Innovation Summit 2026 (London)

Featured Start-up presenting Water OS and SeaFect to world-class aquaculture investors.

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World Aquaculture Society (WAS 2026 Singapore)

Scientific oral presentation accepted on autonomous water stabilization and zero-exchange trials.

GET IN TOUCH

Co-Creation & Paid Sample Inquiry

Connect with our team to request SeaFect paid evaluation samples, discuss joint R&D projects in agriculture, fisheries, sanitation, or novel materials compounding. We respond within two business days.